Instantaneous Topical Drug Quantification using a 3D Printed Microfluidic Device and Coherent Raman Imaging
Cutaneous drug concentration quantification after topical application remains an active, yet challenging research area for topical drug development. Macroscale approaches quantify cutaneous pharmacokinetics 30 minutes to hours after application and miss rapid temporal and spatial dynamics, vital to comprehending drug disposition. We have developed a 3D-printed applicator coupled with an inverted microscope and a rapidly-tunable fiber optic laser to quantify active pharmaceutical ingredients via sparse spectral sampling stimulated Raman scattering.
Highlights
Developed a 3D-printed applicator to monitor active pharmaceutical ingredients immediately after topical application.
- S4RS and the 3D printed applicator captures differences in exposure in frozen skin
- 3D printed applicator requires low formulation volume, is low-cost, and achieves low sample drift
- Propylene Glycol provides more rapid permeation of Rux compared to DGME
The 3D-printed applicator is cost-effective (< $0.70/applicator) and utilizes a small formulation volume (20 µL). Ruxolitinib was formulated in two known permeation enhancers (propylene glycol and diethylene glycol monoethyl ether) that are known to display different permeation profiles to validate device capabilities. Results indicated that the applicator enabled relative-concentration monitoring immediately following drug product application. This approach has significant potential for investigating novel excipients, active pharmaceutical ingredients, and formulations to understand the permeation and biodistribution of these compounds.
Download the full article as PDF here Instantaneous Topical Drug Quantification using a 3D Printed Microfluidic Device and Coherent Raman Imaging
or read it here
Benjamin A. Kuzma, Dandan Tu, Avery Goss, Fotis Iliopoulos, Julian Byrne Slade, Anna Wiatrowski, Amin Feizpour, Conor L. Evans, Instantaneous Topical Drug Quantification using a 3D Printed Microfluidic Device and Coherent Raman Imaging, OpenNano, 2023, 100151, ISSN 2352-9520, https://doi.org/10.1016/j.onano.2023.100151.
Read more on Overview of Pharmaceutical 3D printing here
