Preparation, Characterization and Stability Study of Eugenol-Loaded Eudragit RS100 Nanocapsules for Dental Sensitivity Reduction
Eugenol is a phenolic compound with numerous biological activities. It is widely used in dentistry to treat toothache and pulpitis. In particular, eugenol may be used for tooth whitening procedures to minimize dental sensitivity in patients. However, eugenol has some disadvantages such as its volatility, its photosensitivity, and immediate effect, which can be avoided by using pharmaceutical nanotechnology. The aim of the present study was to obtain, characterize, quantify, and evaluate the physicochemical stability of eugenol-loaded Eudragit RS100 nanocapsules.
Highlights
Eugenol-loaded Eudragit RS100 nanocapsules were suitably obtained and characterized.
- A simple and fast UPLC/MS-MS method for determining eugenol in Eudragit RS100 nanocapsules was developed and validated.
- Eugenol-loaded Eudragit RS100 nanocapsules showed physical stability after 60 days of preparation.
Eugenol-loaded Eudragit RS100 nanocapsules may be further used for reducing dental sensitivity.
The nanocapsules (NCs) were prepared by interfacial deposition of the preformed polymer method. The NCs were characterized through morphological and spectroscopic studies. The encapsulation efficiency was achieved by quantifying the non-encapsulated eugenol using a previously developed and validated analytical method. The physicochemical stability of NCs was assessed at predetermined time intervals for 90 days after preparation. The nanocapsules were successfully prepared by the chosen method and had a predominantly spherical shape with a smooth surface. The mean size, the polydispersion index, and the zeta potential were in agreement to literature data. Infrared spectra ensured that the nanoencapsulation process did not result in chemical reactions between the drug and the polymer. The formulations showed encapsulation efficiency higher than 90% and remained stable after 60 days of preparation. Thus, eugenol-loaded Eudragit RS100 nanocapsules may be further considered as an alternative formulation for dental sensitivity in order to provide a controlled release, a decreased toxicity, and a better dental sensitivity relief.
Materials
Eugenol 99% pure (Sigma-Aldrich, St. Louis, MO, USA), Eudragit RS100 (Mw = 150,000 g/mol, Röhm, Darmstadt, Alemanha), sorbitan monooleate (Span 80, Oxiteno, Mauá, Brazil), polysorbate 80 (Tween 80, Delaware, Porto Alegre, Brazil), medium chain triglycerides (MCT, 99% pure, Focus Química, São Paulo, Brazil), lactose monohydrate (LAC, Biotec Produtos Químicos, São José dos Pinhais, Brazil), acetone (99.9% pure, Vetec Química, Rio de Janeiro, Brazil), acetonitrile HPLC grade (J.T. Baker, Phillipsburg, NJ, USA), Formic acid (Biotec Produtos Químicos, São José dos Pinhais, Brazil) were used as received. Water was purified in a Milli-Q Plus water purification system (Millipore, Bedford, MA, USA).
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Vilela, A. P., Ferreira, L., Biscaia, P. B., Silva, K. L. da ., Beltrame, F. L., Camargo, G. dos A., Rezende, M., Nadal, J. M., Novatski, A., Manfron, J., Loguercio, A. D., & Farago, P. V.. (2023). Preparation, Characterization and Stability Study of Eugenol-Loaded Eudragit RS100 Nanocapsules for Dental Sensitivity Reduction. Brazilian Archives of Biology and Technology, 66(spe), e23230300.
https://doi.org/10.1590/1678-4324-ssbfar-2023230300