Hayashibara Maltose PH – For Culture Media
Hayashibara ― A world leading supplier of pharmaceutical grade Maltose PH
Founded as a starch syrup manufacturer in 1883, Hayashibara has grown into a company with strong research and development expertise, engaging in original and creative research. Utilizing our technological background in biotechnology and in-depth knowledge of saccharide production, Hayashibara became the first company in the world to succeed in the mass production of “Trehalose”, a unique disaccharide recognized as a beneficial excipient for the biopharmaceutical industry. In addition to trehalose, Hayashibara has been supplying for many years other high purity ingredients to the pharmaceutical industry worldwide.
Maltose PH – For Culture Media
What is MALTOSE PH?
- Maltose is a reducing disaccharide consisting of two glucose molecules linked by an α-1,4 bond
- Maltose is manufactured from starch by enzyme technology
- Maltose PH is highly purified crystalline maltose monohydrate with low endotoxin
Effect of Maltose PH supplementation on antibody production by CHO cell
Methods
- CHO-K1 cells were inoculated at 0.3 × 106 cells/mL in duplicates in a DMEM/F12-based protein free chemically defined medium (PFCDM) supplemented with 2 g/L D-(+)-glucose, or glucose with an additional 10 g/L MALTOSE PH in single-use Erlenmeyer flasks.
- The cultures were incubated in a humidified incubator at 37℃, 8% CO2 and a rotation speed of 110 rpm. Cell culture supernatants were collected daily on days 0-5 and 7, and monoclonal IgG antibody (anti-Her2) titers were determined by nephelometry using IMMAGE 800 (Beckman Coulter).
- Two g/L of glucose was chosen as the base glucose concentration because this will allow for a premature but controlled cell growth constraint due to glucose depletion on Day 4.
- IgG titer reached a maximum of 340 mg/L on Day 5 in the culture containing 2 g/L glucose as a carbon source.
- IgG production continued in the MALTOSE PH supplemented cultures Days 5 through 7 to reach 600 mg/L.
Effect of Maltose supplementation on cell viability and viable density
Methods
- The CHO-K1 cells were inoculated and cultured as described in the previous experiment.
- Viable cell density (VCD) and culture viability were analyzed by Vi-Cell XR Cell Viability Analyzer (Beckman Coulter, Brea, CA) according to manufacturer’s instructions.
Summary
- MALTOSE PH can be used by the CHO-K1 cells as a carbon source to maintain culture viability and IgG production upon glucose depletion.
- MALTOSE PH supplementation to the CHO-K1 cell culture in addition to glucose increased the IgG titer compared to the culture with only glucose as a carbon source.
- MALTOSE PH supplementation along with glucose improved the growth of CHO-K1 cell compared to the culture without MALTOSE PH supplementation.
Product Information – HIGH PURITY MALTOSE
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Source: Hayashibara brochure “Hayashibara Maltose PH – culture media brochure”
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