Hayashibara Trehalose SG – For Culture Media Use brochure

Hayashibara Trehalose SG - For Culture Media Use_Fig. 3

Hayashibara ― A world leading supplier of pharmaceutical grade Trehalose

Founded as a starch syrup manufacturer in 1883, Hayashibara has grown into a company with strong research and development expertise, engaging in original and creative research. Utilizing our technological background in biotechnology and in-depth knowledge of saccharide production, Hayashibara became the first company in the world to succeed in the mass production of “Trehalose”, a unique disaccharide recognized as a beneficial excipient for the biopharmaceutical industry. In addition to trehalose, Hayashibara has been supplying for many years other high purity ingredients to the pharmaceutical industry worldwide.

TREHALOSE SG – For Culture Media Use

What is TREHALOSE SG？

TREHALOSE SG is a dihydrous, crystalline and non-reducing disaccharide consisting of two glucose molecules linked by an α,α-1,1 bond.
TREHALOSE SG is white crystalline powder.
TREHALOSE SG is very soluble in water and very heat stable.
TREHALOSE SG is an injectable grade of pharmaceuticals and is monographed as being low endotoxin.

Evaluation of the suppression of protein aggregation in CHO cell culture

Materials & Methods (1)

Protein solution of a bispecific antibody (bispecific
single-chained diabody with Fc, scDb-Fc, 0.8 mg/mL) was prepared with or without addition of trehalose (200 mM) in culture medium.
Circular dichroism (CD) spectroscopy of the scDb-Fc protein contained in the supernatant was measured.

Materials & Methods (2)

The scDb-Fc (0.8 mg/mL) solution prepared in the same manner as described in M & M (1) was heat-treated at 60℃ for 5 minutes.
The precipitate was removed by centrifugation.
The soluble fraction of scDb-Fc protein in the supernatant was measured.

Conclusion

It was possible to culture the antibody-producing CHO cell line in the presence of TREHALOSE SG.
TREHALOSE SG can suppress antibody aggregation, especially the formation of high-order aggregates, during the cell culture process.
Use of TREHALOSE SG appears to increase efficiency of functional antibody production.

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Source: Hayashibara brochure “Trehalose SG – For Culture Media Use”

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