Microwave-assisted β-cyclodextrin/chrysin inclusion complexation: An economical and green strategy for enhanced hemocompatibility and chemosensitivity in vitro
Highlights
- • 1:1 solid inclusion complex (IC) of chrysin with β-cyclodextrin prepared.
- • Microwave irradiation method is time-saving and environmentally benign.
- • Microwaved IC has highest drug content, solubility and hemocompatibility.
- • Microwaved IC displayed superior chemosensitivity against MCF-7 cells.
Chrysin (5, 7-dihydroxyflavone) (CHR), a wonder flavonoid that nature has bestowed upon us, is endowed with a broad spectrum of pharmacological properties. Nonetheless, the practical uses of CHR are greatly impeded owing to its poor aqueous solubility and low oral bioavailability. Inclusion complexes (ICs) in β-cyclodextrin (β-CD) have been an effectual strategy for the same and herein, CHR/β-CD solid ICs were accomplished by diverse approaches.
The inclusion phenomenon was affirmed from different spectroscopic techniques. Molecular dynamic simulation studies ascertained the high stability of CHR/β-CD IC. The microwave irradiation method, which is environmentally more benign, was found to be the optimum wherein the product (MW) exhibited better characteristics in terms of drug content and dissolution.
In vitro hemolytic assay revealed no adverse effect on RBC morphology and the toxicity of CHR was significantly reduced by employing MW. Furthermore, the MW product demonstrated improved chemosensitivity potency in terms of intracellular uptake and cytotoxicity against MCF-7cells in comparison to pristine CHR. Our findings unambiguously validate the efficacy of MW as a superior drug formulation for pharmaceutical applications of CHR delivery.
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Author links open overlay panelSubhraseema Das, Subhrajit Mohanty, Jitendra Maharana, Soumya R. Jena, Jasmine Nayak, Usharani Subuddhi,
Microwave-assisted β-cyclodextrin/chrysin inclusion complexation: An economical and green strategy for enhanced hemocompatibility and chemosensitivity in vitro, Journal of Molecular Liquids, Volume 310, 2020, 113257, ISSN 0167-7322, https://doi.org/10.1016/j.molliq.2020.113257.