Analysis of phospholipids and triacylglycerols in intravenous lipid emulsions
Intravenous lipid emulsions (ILEs) are used for parenteral nutrition, providing a vital source of essential fatty acids and concentrated energy for patients who are unable to absorb nutrients via the digestive track. They are commonly used to treat local and non-local anesthetic toxicity, and lipophilic drug overdose. ILE are composed of natural lipids, and the composition of these natural lipids can be varied based on their source. The lipids are susceptible to hydrolytic degradation with time, resulting various lipid degradation products such as Lysophosphatidylcholines (LPs), affecting the actual composition of nutrients in the formulation. As a result, the identification and quantification of lipid components, including degradation products, in ILEs are crucial in quality control.
Highlights
- UPLC-QTOF-MS method was developed to determine the lipid composition in intralipid nutritional formulation.
- Of the 47 lipids identified, 34 were phospholipids (PLs) and the others were triacylglycerols.
- Most of the phospholipids detected were phosphocholines (PC) and lysophosphatidylcholines (LPC).
- A total of 9 LPCs, 18 PCs, 6 phosphoethanolamines (PEs), and 1 sphingomyelin (SM) were detected.
In this study, lipids from different batches of ILE Intralipid® 20%, were separated and identified using a UHPLC-ESI-QTOF system and SimLipid® high throughput lipid identification software. Out of 47 lipids identified, 34 were phospholipids (PLs) and the others were triacylglycerols (TAGs). Most of the phospholipids detected were phosphatidylcholines (PC) and Lysophosphatidylcholines (LPC). A total of 9 LPCs, 18 PCs, 6 phosphoethanolamines (PEs), and 1 sphingomyelin (SM) were identified. The LPCs concentration changed with the manufacturing date and storage time. This UHPLC method enabled the identification and quantification of lipids and their decomposition products in complex ILE emulsion mixtures on a single 20-minute chromatographic run.
Download the full article as PDF here Analysis of phospholipids and triacylglycerols in intravenous lipid emulsions
or read it here
Bijay Banstola, Prabhath L. Gamage, Wenlei Jiang, Thilak Mudalige, Analysis of phospholipids and triacylglycerols in intravenous lipid emulsions, Journal of Pharmaceutical and Biomedical Analysis, Volume 222, 2023, 115112, ISSN 0731-7085, https://doi.org/10.1016/j.jpba.2022.115112.