Novel Collagen-Polyphenols-Loaded Silica Composites for Topical Application

Lesions can affect skin functions and cause a simple issue, such as dehydration, or more challenging complications, such as bacterial infections. The purpose of this study was to design composites for topical application that can prevent and/or assist in bacterial infections and support cell regeneration using natural components. A polyphenolic extract obtained from Salvia officinalis was embedded in functionalized mesoporous silica nanoparticles for better stability, followed by their distribution into a collagen porous scaffold. The resulting polyphenols-loaded MSN exhibited enhanced antibacterial activity and good cytocompatibility. Improved thermal stability of the collagen porous scaffold was obtained due to the presence of the functionalized MSN. For the first time, collagen-polyphenols-loaded silica composites were reported in the literature as potential wound dressings. The newly developed composites showed excellent sterility.

2.1. Materials

Tetraethyl orthosilicate (TEOS, Fluka, Seelzer, Germany), triethanolamine (TEA, Sigma-Aldrich Co., Merck Group, Darmstadt, Germany), cetyltrimethylammonium chloride (CTAC, 25 %wt in water, Sigma-Aldrich Co.), poly(ethylene glycol) methyl ether (PEG, average M_n 550, Sigma-Aldrich), (3-aminopropyl)triethoxysilane (APTES, 99% Sigma-Aldrich), 3-(triethoxysilyl)propionitrile (CETES, 97%, Sigma-Aldrich), ammonium chloride (Sigma-Aldrich), toluene (Riedel de Haen, Honeywell Riedel-de Haën, Seelzer, Germany), acetone (Sigma, Merck Group, Darmstadt, Germany), absolute ethanol (Sigma-Aldrich), 36.5–38% hydrochloric acid, sulfuric acid 98% (Merck), acetic acid (>99.7%, Sigma-Aldrich Co.), Folin–Ciocalteu (Sigma-Aldrich), 2,2′–azino–bis(3–ethylbenzothiazoline–6–sulphonic acid) (ABTS, Sigma-Aldrich Co.), and potassium persulphate (K₂S₂O₈, Sigma-Aldrich Co.) were used as received. Ultrapure water (Millipore Direct-Q3 UV water system, version Q3 UV, product no. C9185, Merck Group, Darmstadt, Germany, equipped with a Biopack UF cartridge) was used for all experiments. Collagen was provided by the National Institute of Research and Development for Biological Sciences, Bucharest, Romania and prepared as previously described [36].

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Deaconu, M.; Prelipcean, A.-M.; Brezoiu, A.-M.; Mitran, R.-A.; Isopencu, G.; Matei, C.; Berger, D. Novel Collagen-Polyphenols-Loaded Silica Composites for Topical Application. Pharmaceutics 2023, 15, 312. https://doi.org/10.3390/pharmaceutics15020312

excipients formulation