Preparation and Characterization of Tacrolimus-Loaded SLNs in situ Gel for Ocular Drug Delivery for the Treatment of Immune Conjunctivitis
Background: The aim of this study is to develop a novel in situ gel of tacrolimus-loaded SLNs (solid lipid nanoparticles) for ocular drug delivery.
Methods: The optimal formulation was characterized by surface morphology, particle size, zeta potential, entrapment efficiency, drug loading and in vitro release behavior. In vivo studies were also conducted to evaluate the pharmacokinetic and pharmacodynamic results.
Results: In this study, TAC-SLNs ISG were prepared using homogenization followed by probe sonication method. The average particle size of TAC-SLNs ISG was observed to be 122.3±4.3 nm. Compared with TAC-SLNs, in situ gel did not increase particle size, and there was no significant difference between them. The results of viscosity measurement showed that TAC SLNs-ISG were typical of pseudo plastic systems and showed a marked increase in viscosity as temperature increased and ultimately formed a rigid gel (32°C). In vitro and in vivo studies illustrated the sustained release model of the drug from TAC-SLNs ISG. Animal model showed that TAC-SLNs ISG had good pharmacodynamics when compared with eye drops and SLNs.
Conclusion: Our results demonstrated that TAC SLNs-ISG had the potential for being an ideal ocular drug delivery system.
Download the full article as a PDF here or read it here
Article Information: Sun K, Hu K. Preparation and Characterization of Tacrolimus-Loaded SLNs in situ Gel for Ocular Drug Delivery for the Treatment of Immune Conjunctivitis. Drug Des Devel Ther. 2021;15:141-150
https://doi.org/10.2147/DDDT.S287721
Materials:
TAC was purchased from Kerui Pharmaceutical Co., Ltd (Fujian, China). Compritol® 888 ATO (glyceryl behenate) and glyceryl monostearate (GMS) were purchased from Gattefossé. Pluronic® F-68 (Poloxamer 188), Pluronic® F-127 (Poloxamer 407) and Tween® 80 were obtained from Sinopharm Chemical Reagent (Shanghai, China). All the other reagents used were of analytical grade and were used without further purification.